Chicoric acid alleviates LPS-induced inflammatory response through miR-130a-3p/IGF-1pathway in human lung A549 epithelial cells

To investigate the effects and potential mechanisms of chicoric acid (CA) on LPS-induced inflammatory response in A549 cells. 0–800 μM CA was added to cells determine toxicity using MTT assay. Then, 2 μg/mL LPS 50 were simultaneously CA. In order miR-130a-3p IGF-1 cells, infected with inhibitor si RNA IGF-1. The levels cytokines such as IL-1β, IL-6, TNF-α measured by real-time RT-PCR enzyme-linked immunosorbent (ELISA) pathway NF-κB expression immunoblot Moreover, a luciferase activity assay used indicate binding site 3′UTR 0–50 had no Thus, we chose for following study. attenuated through decreasing increasing IGF-1/IGF-1R expression. Inhibition reduced restored induced LPS. Furthermore, results indicated that directly targeted regulate response. alleviates miR-130a-3p/IGF-1pathway